Years of collective research conducted on the N-O Pro delivery system substantiate consistent N-O production, release, and uptake within minutes as the lozenge dissolves in the mouth. Nitric oxide has a short half-life and is oxidized quickly back to nitrite which helps build nitrite reserves. Many factors contribute to the rate at which a person can build nitrite reserves. As a steady-state is eventually created in nitrite concentration in the body, the salivary indicator strip will register a color change indicating improvement in N-O bioavailability. Yet, N-O repletion is an individual process.